Gru and Lucy and their girls—Margo, Edith and Agnes—welcome a new member to the Gru family, Gru Jr., who is intent on tormenting his dad. Gru also faces a new nemesis in Maxime Le Mal and his femme fatale girlfriend Valentina, forcing the family to go on the run.
Lincoln and the Louds are ecstatic to welcome their new Gran-Gran, Myrtle, into the family with a tropical wedding celebration; but the festivities are cut short when an old nemesis from Myrtle's secret agent past appears on the island.
While working underground to fix a water main, Brooklyn plumbers—and brothers—Mario and Luigi are transported down a mysterious pipe and wander into a magical new world. But when the brothers are separated, Mario embarks on an epic quest to find Luigi.